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Under these conditions, embedded cells proliferate and self-organize into 3D organoid structures that, with many systems, can be passaged and maintained indefinitely.Additionally, these cultures have shown remarkable genetic stability during passaging; sequencing liver organoids clonally expanded from a single liver progenitor cell through whole genome sequencing revealed only one synonymous base substitution after three months passage.

For these and other applications, organoid cultures constitute highly informative complementary approaches to the existing 2D-culture methods and animal model systems.

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